DFCA DA
| Code | Size | Price |
|---|
| CDX-C0042-M100 | 100 mg | £128.00 |
Quantity:
| CDX-C0042-G001 | 1 g | £682.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4 °C
Documents
Further Information
Alternate Names/Synonyms:
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate
Appearance:
White to off-white powder.
CAS:
127770-45-0
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H315, H319, H335
Long Description:
Chemical. CAS: 127770-45-0. Formula: C25H14Cl2O9. MW: 529.28. Synthetic. Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH.
MDL:
MFCD00037454
Molecular Formula:
C25H14Cl2O9
Molecular Weight:
529.28
Package Type:
Vial
Precautions:
P261, P305, P351, P338
Product Description:
Non-activated, amine-reactive fluorescent probe. Mixture of 5/6-isomers of CDCFDA that can be used in biomolecules conjugation. 5(6)-CDCFDA is an amine?reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. 5(6)-CDCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: lambdaex=504nm, lambdaem=529nm.
Purity:
>85% (HPCE)
Signal word:
Warning
SMILES:
CC(O)=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2
Solubility Chemicals:
Soluble in DMSO, DMF or methanol.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
References
(1) J.P. Kehrer & T. Paraidathathu; Free Rad. Res. Commun. 16, 217 (1992) | (2) Q.P. Lloyd, et al.; J. Biol. Chem. 270, 22445 (1995) | (3) A.L. Inselman, et al.; Curr. Microbiol. 38, 295 (1999) | (4) M.J. Zamek-Gliszczynski, et al.; J. Pharmacol. Exp. Therap. 304, 801 (2003) | (5) L. Li, et al.; Free Radic. Res. 42, 354 (2008) | (6) M. Medhora, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) | (7) S. Siissalo, et al.; Eur. J. Pharm. Biopharm. 71, 332 (2009) | (8) A.I. Pogue, et al.; Int. J. Mol. Sci. 13, 9615 (2012) | (9) J. Roh, et al.; Redox Biol. 11, 254 (2016) | J.L. Roh, et al.; Cancer Lett. 381, 96 (2016)
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| DCFDA | CDX-D0063 | Chemodex | Summary Details | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||