Dihydrofluorescein diacetate
| Code | Size | Price |
|---|
| CDX-D0122-G001 | 1 g | £109.00 |
Quantity:
| CDX-D0122-G010 | 10 g | £383.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20 °C
Documents
Further Information
Appearance:
Solid.
CAS:
35340-49-9
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)
InChiKey:
YKSJJXGQHSESKB-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 35340-49-9. Formula: C24H18O7. MW: 418.4. Synthetic. Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).
MDL:
MFCD00005056
Molecular Formula:
C24H18O7
Molecular Weight:
418.4
Package Type:
Vial
Product Description:
Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).
Purity:
>97% (NMR)
SMILES:
CC(=O)OC1=CC2=C(C=C1)C(C1=C(O2)C=C(OC(C)=O)C=C1)C1=C(C=CC=C1)C(O)=O
Solubility Chemicals:
Soluble in chloroform.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) P.P. Williams & R.L. Stolzenberg; Applied Microbiology 23(4), 745-9 (1972) | (2) S.L. Hempel, et al.; Free Radical Biology & Medicine 27(1/2), 146-159 (1999) | (3) Y. O'Malley et al.; Free Radical Biology & Medicine 36(1), 90-100 (2004)
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