5(6)-FITC
| Code | Size | Price |
|---|
| CDX-F0010-M100 | 100 mg | £79.00 |
Quantity:
| CDX-F0010-M250 | 250 mg | £102.00 |
Quantity:
| CDX-F0010-G001 | 1 g | £188.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20 °C
Documents
Further Information
Alternate Names/Synonyms:
Fluorescein 5(6)-isothiocyanate
Appearance:
Yellow to orange powder.
CAS:
27072-45-3
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS08
Handling Advice:
Protect from light and moisture.
Hazards:
H334
InChi:
InChI=1S/C20H12O5.C2H3NS/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;1-3-2-4/h1-10,21-22H;1H3
InChiKey:
QRQQVJDEAPQYJW-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 27072-45-3. Formula: C21H11NO5S. MW: 389.38. Synthetic. Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.
MDL:
MFCD00005063
Molecular Formula:
C21H11NO5S
Molecular Weight:
389.38
Package Type:
Vial
Precautions:
P261, P342, P311
Product Description:
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.
Purity:
>95% (HPLC)
Signal word:
Danger
SMILES:
CN=C=S.OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=C(O2)C=C(O)C=C1
Solubility Chemicals:
Soluble in aqueous buffers (pH > 6).
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) R.W. Dirks et al.; J. Histochem. Cytochem 38, 467 (1990) | (2) S. Wu et al.; J. Chromatogr 480, 141 (1989) | (3) J.C. Vera et al.; Anal. Biochem 174, 38 (1988) | (4) E.T. Thean et al.; Anal. Biochem 177, 263 (1989) | (5) D.S. Perlin et al.; J. Biol. Chem 259, 9532 (1984) | (6) K.G. Mann et al.; Meth. Enzymol. 26, 28 (1972)
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