Merocyanin 540

MC-540

Deep violet powder.

62796-23-0

32160000

liquid

GHS07

Protect from light and moisture.

H315, H319, H335

InChI=1S/C26H33N3O6S2.Na/c1-3-5-16-28-24(30)20(25(31)29(26(28)36)17-6-4-2)12-7-10-15-23-27(18-11-19-37(32,33)34)21-13-8-9-14-22(21)35-23;/h7-10,12-15H,3-6,11,16-19H2,1-2H3,(H,32,33,34);/q;+1/p-1/b10-7+,23-15-;

OSQUFVVXNRMSHL-LTHRDKTGSA-M

Chemical. CAS: 62796-23-0. Formula: C26H32N3NaO6S2. MW: 569.67. Synthetic. Sensitive probe for membrane potential (Ex/Em: 555/578nm in methanol). Selective staining of immature hemopoietic cells in flow cytometry.

MFCD00013438

C26H32N3NaO6S2

569.67

Vial

P261, P305, P351, P338

Merocyanine 540 (MC-540) is an anionic, lipophilic sensitive fluorescent probe for membrane potential. It is a sodium salt of sulfonic acid, with a chain of four conjugated methine groups, a substituted benzoxazole ring at one end, and substituted thiobarbituric acid at the other end. MC-540 was originally developed as a photosensitizer for photographic emulsions. However, its main importance today is in biology and medicine. It is widely used as a biological probe in membrane studies (fluorescent probe for studies of membrane systems, optical probe for transmembrane potentials), for characterization of the multidrug resistance phenotype, for photo-induced cytotoxicity as a highly effective drug against human malignant cells (photodynamic purging of neoplastic cells in bone marrow explants - leukemia, treatment of lymphomas and metastatic neuroblastomas) and other diseases (malaria, Herpes simplex, HIV). MC-540 has been shown to be a selective stain for leukemic and immature hemopoietic cells in flow cytometry. Spectral Data: Ex/Em: 560/579nm (see Reference 10 for excitation of different solvents). MC-540 is spectrally similar to Alexa Fluor 546, Cy3 (Cyanine-3), Sulforhodamine B, TAMRA and Sulforhodamine B sulfonyl chloride.

>90% (HPLC)

Warning

[Na+].CCCCN1C(=O)C(=CC=CC=C2/OC3=CC=CC=C3N2CCCS([O-])(=O)=O)C(=O)N(CCCC)C1=S

Soluble in DMSO, ethanol, methanol or water.

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at -20°C.