Resazurin sodium salt

Alamar Blue; Vybrant; UptiBlue; 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

Black-Violet solid.

62758-13-8

32160000

liquid

GHS07

Protect from light and moisture.

H315, H319, H335

InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

IVGPGQSSDLDOLH-UHFFFAOYSA-M

Chemical. CAS: 62758-13-8. Formula: C12H6NNaO4. MW: 251.17. Synthetic. Fluorescent reagent.

MFCD00005036

C12H6NNaO4

251.17

Vial

P261, P305, P351, P338

Resazurin (also known as Alamar Blue), is the N-oxide of the fluorescent dye resorufin. It is useful for detecting reductive activities in cells and has been widely used for measuring cell proliferation and mitochondrial metabolic activity. It is used as a quantifiable detection agent for enzyme activity assays and as an oxidation-reduction indicator in cell proliferation, cell viability and cytotoxicity assays. Resazurin is a non-toxic, water-soluble and redox-sensitive dye that is non-fluorescent until it is reduced to the highly red fluorescent resorufin (lambdaEx/lambdaEm: 563/587 nm). Usually, NADPH or NADH is the reductant that converts resazurin to resorufin in the presence of diaphorase as the enzyme. Thus, resazurin can be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin/diaphorase/NADPH system can be used to detect any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH.

~80% (Dye Content) (UV/Vis). Suitable for cell culture application.

Warning

[Na+].[O-]C1=CC=C2C(OC3=CC(=O)C=CC3=[N+]2[O-])=C1

Soluble in water (1mg/ml).

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at +4°C.