DHODH Antibody

ProSci
Product Code: PSI-29-658
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-29-658-100ul100ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store DHODH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

Further Information

Additional Names:
DHODH, DHOdehase, URA1, POADS
Application Note:
DHODH antibody can be used for detection of DHODH by ELISA at 1:312500. DHODH antibody can be used for detection of DHODH by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Kimura, K., (2006) Genome Res. 16 (1), 55-65.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DHODH.
NCBI Gene ID #:
1723
NCBI Official Name:
dihydroorotate dehydrogenase (quinone)
NCBI Official Symbol:
DHODH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_001352
Protein GI Number:
45006951
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane
Swissprot #:
Q02127
User NOte:
Optimal dilutions for each application to be determined by the researcher.