GABAA Receptor Antibody

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ProSci
Product Code: PSI-50-210
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-210-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
GABAA Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat brain lysates from wild type (Control) and alpha3-knockout (alpha3-K/O) animals showing specific immunolabeling of the ~51k alpha3-subunit of the GABAA receptor. The labeling was absent from a lysate prepared from alpha3-knockout animals.
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Western blot of rat brain lysates from wild type (Control) and alpha3-knockout (alpha3-K/O) animals showing specific immunolabeling of the ~51k alpha3-subunit of the GABAA receptor. The labeling was absent from a lysate prepared from alpha3-knockout animals.

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat and mouse tissue.
Background:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl? channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P?ltl et al., 2003).
Background References:
  • McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α1-subtype. Nature Neurosci 3:587-592.
  • Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
  • Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ2-subunit. Biochem Pharmacol 68:1621-1629.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Fusion protein from N-terminal region of the a3-subunit of rat GABAA receptor.
NCBI Gene ID #:
24947
NCBI Official Name:
gamma-aminobutyric acid (GABA) A receptor, alpha 3
NCBI Official Symbol:
Gabra3
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51
Protein Accession #:
P20236
Protein GI Number:
120761
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
P20236
User NOte:
Optimal dilutions for each application to be determined by the researcher.