GABA A Receptor beta 3 Antibody
| Code | Size | Price |
|---|
| PSI-XPS-2012-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Human
- Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Documents
Further Information
Application Note:
GABAA Receptor antibody is specific for the 51 to 56k GABAA-Receptor β3-Subunit in rat brain membrane fractions. Antibody dilutions and tissue load should be based on tissue type and expected expression level. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA-Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha subunits of the receptor. Lastly, phosphorylation of beta subunits of the receptor has been shown to modulate GABAA-R function.
Background References:
- Kittler, J.T., McAinsh, K., Moss, S.J. "Mechanisms of GABA A receptor assembly and trafficking - Implications for the modulation of inhibitory neurotransmission," Mol. Neurobiol 26 (2002) 2251 - 268.
- Slany, A., Zezula, J., Tretter, V., and Sieghart, W. "Rat β3-subunits expressed in human embryonic kidney 293 cells form high affinity [35S]t-butylbicyclophosphorothionate binding sites modulated by several allosteric ligands of γ-aminobutyric acid type A receptors," Mol. Pharmacol. 48 (1995), 385-391.
- Whiting, P.J., McKernan, R.M., Wafford, K.A. "Structure and pharmacology of vertebrate, GABAA receptor subtypes." In: International Review of Neurobiology (Bradley, R.J., Harris R.A., eds.), pp 95. San Diego: Academic Press (1995).
- Olsen, R.W., Tobin, A.J. "Molecular biology of GABAA receptors," FASEB 4 (1990) 1469 - 1480.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
GABAA Receptor b3 (CT) polyclonal antibody was raised against a fusion protein from the amino acid sequence representing the cytosolic loop of rat GABAA-Receptor b3-Subunit.
NCBI Gene ID #:
24922
NCBI Official Name:
gamma-aminobutyric acid (GABA) A receptor, beta 3
NCBI Official Symbol:
Gabrb3
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53
Protein Accession #:
P63079
Protein GI Number:
52000955
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
P63079
User NOte:
Optimal dilutions for each application to be determined by the researcher.