Metabotropic Glutamate Receptor 1a Antibody

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ProSci
Product Code: PSI-50-101
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-101-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Metabotropic Glutamate Receptor 1a antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C. This product is stable at -20˚C for at least 1 year.

Images

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Western blot of 10ug of HEK 293 cells expressing mGluR1a and mGluR5 showing specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a.
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Western blot of 10ug of HEK 293 cells expressing mGluR1a and mGluR5 showing specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a.

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Further Information

Application Note:
Metabotropic Glutamate Receptor 1a antibody allows for strong and specific immunolabeling of ~125k mGluR1a and the ~250k mGluR1a dimer in Western blots of rat brain at antibody dilution of 1:1000. Immunolabeling blocked by preadsorption of antibody with the C-terminal peptide immunogen. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). Rabbit anti-mGluR recognizes mGluR1a in rat brain. When internally tested under ideal conditions the working dilutions were 1:500 for IHC and 1:1000 for DB and WB.
Background:
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase.
Background References:
  • Kim, S.J., Kim, Y,S, Yuan, J.P., Petralia, R.S., Worley, P.F. and Linden, D.J., "Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1", Nature (London) 426 (2003) 285 - 291.
  • Rammes, G., Palmer, M., Eder, M., Dodt, H.U., Zieglgansberger, W. and Collingridge, G.L., "Activation of mGlu receptors induces LTD without affecting postsynaptic sensitivity of CA1 neurons in rat hippocampal slices," J. Physiol 546 (2003) 455 - 460.
  • Ferr?, S., Karcz-Kubicha, M., Hope, B.T., Popoli, P., Burgue?o, J., Guti?rrez, M.S., Casad?, V., Fuxe, K., Goldberg, S.R., Lluis, C. Franco, R. and Ciruela, F., "Synergistic interaction between adenosine A2A and glutamate mGlu5 receptors: Implications for striatal neuronal function," Proc. Natl. Acad. Sci. USA 99 (2002) 11,940-11,945.
  • Huber, K.M., Kayser, M.S., and Bear, M.F., "Role for rapid dendritic protein synthesis in hippocampal mGluR-dependent long-term depression," Science 288 (2000) 1254 - 1256.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
mGluR 1a polyclonal antibody was raised against a peptide from the C-terminal region of mGluR1a.
NCBI Gene ID #:
24414
NCBI Official Name:
glutamate receptor, metabotropic 1
NCBI Official Symbol:
Grm1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125/250
Protein Accession #:
P23385
Protein GI Number:
121300
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
P23385
User NOte:
Optimal dilutions for each application to be determined by the researcher.