NMDA NR1 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-XPS-2019-15ug | 15ug | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
NMDA NR1 Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl water, the antibody should be aliquot and stored at -20˚C. This product is stable at -20˚C for at least 1 year and can be shipped ambient domestic or inter
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Further Information
Application Note:
NMDA NR1 Subunit antibody allows for strong and specific immunolabeling of the 120k NR1 protein in Western blots of rat brain hippocampal homogenate. Applications include Dot Blots (DB), Immunoprecipitation (IP) and Western Blots (WB). Mouse anti-NMDA NR1 Subunit recognizes NR1 in human, mouse and rat brain lysates. When internally tested under ideal conditions the working dilutions were 1:300 for DB and WB, and 3 μg per 200 μg lysate (will quantitatively precipitate all the NMDA NR1 in 200 μg of rat brain lysate).
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer?s disease, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR1 subunit of the receptor has been associated with synaptic plasticity.
Background References:
- Luo, J.H., Wang, Y.H., Yasuda, R.P., Dunah, A.W. and Wolfe, B.B., "The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B)," Mol. Pharmacol. 51 (1997) 79 - 86.
- Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci., 5 (2002) 27 - 33.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
NMDA NR1 Subunit monoclonal antibody was raised against a fusion protein from AA 1 - 564 of NMDA NR1.
NCBI Gene ID #:
24408
NCBI Official Name:
glutamate receptor, ionotropic, N-methyl D-aspartate 1
NCBI Official Symbol:
Grin1
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
PREDICTED MOLECULAR WEIGHT:
120
Protein Accession #:
P35439
Protein GI Number:
548379
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
P35439
User NOte:
Optimal dilutions for each application to be determined by the researcher.