eIF4B Antibody

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ProSci
Product Code: PSI-79-242
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-242-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).
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Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).
Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).

Documents

Further Information

Additional Names:
EIF-4B, PRO1843, ESR, NR3A1,
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Background References:
  • Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. and Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
eIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1975
NCBI Official Name:
eukaryotic translation initiation factor 4B
NCBI Official Symbol:
EIF4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_001408.2
Protein GI Number:
50053795
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.
Swissprot #:
P23588