HDAC4/HDAC5/HDAC9 Antibody
| Code | Size | Price |
|---|
| PSI-79-244-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A, CYK18, PIG46, HD4/HD5/HD9,
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
Background References:
- Cress, W. D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Vigushin, D. M. and Coombes, R. C. (2004) Curr. Cancer Drug Targets 4, 205-218. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody was raised against a peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S (p) -EP) derived from Human HDAC4/HDAC5/HDAC9.
Modifications:
Phospho-Specific
NCBI Gene ID #:
9759 / 10014 / 9734
NCBI Official Name:
histone deacetylase 4 / histone deacetylase 5 / histone deacetylase 9
NCBI Official Symbol:
HDAC4 HDAC5 HDAC9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
140, 124 kDa
Protein Accession #:
NP_006028.2 / NP_001015053.1 / NP_055522.1
Protein GI Number:
153085395 / 62750349 / 7662280
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
Swissprot #:
P56524 Q9UQL6 Q9UKV0