HER2 Antibody

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ProSci
Product Code: PSI-79-140
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-140-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1248).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248).
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Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1248).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248).

Documents

Further Information

Additional Names:
NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, AMPK1, C-erbB-2, ErbB2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
Background References:
  • Yuan, Lu Qian, Ming Shi, et al. (2008) HER2-dependent MMP-7 expression is mediated by activated STAT3 Guogang Cellular Signalling, 20:1284?1291.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HER2 (Phospho-Tyr1248) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y (p) -L-G) derived from Human HER2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2064
NCBI Official Name:
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
NCBI Official Symbol:
ERBB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
185 kDa
Protein Accession #:
NP_001005862.1
Protein GI Number:
54792098
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1248.
Swissprot #:
P04626