HSP27 Antibody

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ProSci
Product Code: PSI-79-181
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-181-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).

Documents

Further Information

Additional Names:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, ASPL, RCC17, TUG, UBXD9, UBXN9, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Involved in stress resistance and actin organization.
Background References:
  • Tremolada L, et al. (2005) Proteomics. 5 (3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3 (2): 115-124. Benn SC, et al. (2002) Neuron. 36 (1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1 (4): 314-322.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3315
NCBI Official Name:
heat shock 27kDa protein 1
NCBI Official Symbol:
HSPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
NP_001531.1
Protein GI Number:
4504517
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of HSP27only when phosphorylated at serine 15.
Swissprot #:
P04792