MEK1 Antibody

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ProSci
Product Code: PSI-79-179
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-179-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MEK1 (Phospho-Ser221).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK1 (Phospho-Ser221).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MEK1 (Phospho-Ser221).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK1 (Phospho-Ser221).

Documents

Further Information

Additional Names:
CFC3, MEK1, MKK1, MAPKK1, PRKMK1, EMS1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Background References:
  • Zebisch A, et al. (2006) Cancer Res; 66 (7): 3401-8. Luciano BS, et al. (2006) J Biol Chem; 279 (50): 52117-23. Wang X, et al. (2003) Oncogene; 22 (1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278 (10): 8118-25. Ling MT, et al. (2002) Oncogene; 21 (55): 8498-505.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MEK1 (Phospho-Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5604
NCBI Official Name:
mitogen-activated protein kinase kinase 1
NCBI Official Symbol:
MAP2K1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_002746.1
Protein GI Number:
5579478
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.
Swissprot #:
Q02750