PKCtheta Antibody

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ProSci
Product Code: PSI-79-230
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-230-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from PC12 cells untreated or treated with PMA using PKCΘ (Phospho-Ser676).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Phospho-Ser676).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCΘ (Phospho-Ser676).
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Western blot analysis of lysed extracts from PC12 cells untreated or treated with PMA using PKCΘ (Phospho-Ser676).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Phospho-Ser676).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PKCΘ (Phospho-Ser676).

Documents

Further Information

Additional Names:
PRKCT, nPKC-theta, HSP90B, HSPC2, HSPCB, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-κ-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Background References:
  • Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253. Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123. Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKCtheta (Phospho-Ser676) antibody was raised against a peptide sequence around phosphorylation site of serine 676 (R-L-S (p) -F-A) derived from Human PKCΘ.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5588
NCBI Official Name:
protein kinase C, theta
NCBI Official Symbol:
PRKCQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_006248.1
Protein GI Number:
5453976
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of PKCΘ only when phosphorylated at serine 676.
Swissprot #:
Q04759