VEGFR2 Antibody

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ProSci
Product Code: PSI-79-143
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-143-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from G-CSF-treated HUVEC and serum-treated HT29 cells using VEGFR2 (Phospho-Tyr951).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Phospho-Tyr951).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using VEGFR2 (Phospho-Tyr951).
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Western blot analysis of lysed extracts from G-CSF-treated HUVEC and serum-treated HT29 cells using VEGFR2 (Phospho-Tyr951).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Phospho-Tyr951).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using VEGFR2 (Phospho-Tyr951).

Documents

Further Information

Additional Names:
FLK1, CD309, VEGFR, VEGFR2, HER3, FLK1, KDR, VGFR2, VGR2, kinase insert domain receptor
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions
Background References:
  • Zeng H, et al. (2001) J Biol Chem. 276 (35): 32714-32719. Dougher M, et al. (1999) Oncogene. 18 (8): 1619-1627.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VEGFR2 (Phospho-Tyr951) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 951 (K-D-Y (p) -V-G) derived from Human VEGFR2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3791
NCBI Official Name:
kinase insert domain receptor (a type III receptor tyrosine kinase)
NCBI Official Symbol:
KDR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
230 kDa
Protein Accession #:
NP_002244.1
Protein GI Number:
11321597
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 951.
Swissprot #:
P35968