Elk-1 Antibody
| Code | Size | Price |
|---|
| PSI-XPS-1008-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚
Documents
Further Information
Application Note:
Immunolabeling of the Elk-1 protein band in a Western blot of recombinant Elk-1 is blocked by the phosphopeptide used as antigen but not by the corresponding non-phospho-Elk-1. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors. Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1. Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus.
Background References:
- Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D., and Martins-Green, M. "MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300." EMBO J. 22 (2003), 281 - 91.
- Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., "Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1," J. Neurosci. 22 (2002) 2054 - 2062.
- Hill, C.S. and Treisman, R. "Transcriptional regulation by extracellular signals: mechanisms and specificity," Cell 80 (1995), 199 - 211.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Elk-1 (Ser383) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser383 of human Elk-1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2002
NCBI Official Name:
ELK1, member of ETS oncogene family
NCBI Official Symbol:
ELK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46
Protein Accession #:
P19419
Protein GI Number:
12643407
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
Elk-1 antibody is specific for ~46k Elk-1 phosphorylated at Ser383 using phospho and dephospho Elk-1 recombinant protein.
Swissprot #:
P19419
User NOte:
Optimal dilutions for each application to be determined by the researcher.