GSK3beta Antibody

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ProSci
Product Code: PSI-79-101
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-101-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3β (Phospho-Ser9).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3β (Phospho-Ser9).
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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using GSK3β (Phospho-Ser9).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using GSK3β (Phospho-Ser9).

Documents

Further Information

Additional Names:
NFKB3, Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Background References:
  • Fan G, et al. (2003) J Biol Chem. 278 (52): 52432-52436. Barry FA, et al. (2003) FEBS Lett. 553 (1-2): 173-178. Welsh, et al. (1996) Trends Cell Biol. 6: 274-279. Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GSK3beta (Phospho-Ser9) antibody was raised against a peptide sequence around phosphorylation site of serine 9 (T-T-S (p) -F-A) derived from Human GSK3β.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2932
NCBI Official Name:
glycogen synthase kinase 3 beta
NCBI Official Symbol:
GSK3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
NP_001139628.1
Protein GI Number:
225903437
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of GSK3β only when phosphorylated at serine 9.
Swissprot #:
P49841