PDK1 Antibody

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ProSci
Product Code: PSI-79-103
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-103-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PDK1 (Phospho-Ser241).
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Western blot analysis of lysed extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using PDK1 (Phospho-Ser241).

Documents

Further Information

Additional Names:
PDK1, PDPK2, PRO0461, NFKB3, PDPK1, PkB kinase, Protein kinase B kinase, hPDK1, kinase PDK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-ζ, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
Background References:
  • Scheid MP, et al. (2005) Mol Cell Biol; 25 (6): 2347-63Chen H, et al. (2001) Biochemistry; 40 (39): 11851-9Sato S, et al. (2002) J Biol Chem; 277 (42): 39360-7Lim MA, et al. (2003) Proc Natl Acad Sci U S A; 100 (24): 14006-11
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PDK1 (Phospho-Ser241) antibody was raised against a peptide sequence around phosphorylation site of serine 241 (A-N-S (p) -F-V) derived from Human PDK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5170
NCBI Official Name:
3-phosphoinositide dependent protein kinase-1
NCBI Official Symbol:
PDPK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
NP_002604.1
Protein GI Number:
4505695
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.
Swissprot #:
O15530