FKHR Antibody

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ProSci
Product Code: PSI-79-154
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-154-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).
<b>Top Image:</b> Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256).

Documents

Further Information

Additional Names:
FKH1, FKHR, FOXO1A, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, FOXO1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Transcription factor.
Background References:
  • Gan L, et al. (2005) J Neurochem; 93 (5): 1209-19. Smith WW, et al. (2005) J Cell Biol; 169 (2): 331-9. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Horn S, et al. (2004) Leukemia; 18 (11): 1839-49.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FKHR (Phospho-Ser256) antibody was raised against a peptide sequence around phosphorylation site of serine 256 (A-A-S (p) -M-D) derived from Human FKHR.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2308
NCBI Official Name:
forkhead box O1
NCBI Official Symbol:
FOXO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 - 82 kDa
Protein Accession #:
NP_002006.2
Protein GI Number:
9257222
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of FKHR only when phosphorylated at serine 256.
Swissprot #:
Q12778