ATF2 Antibody

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ProSci
Product Code: PSI-XPS-1002
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1002-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490,498.
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Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490,498.

Documents

Further Information

Application Note:
The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.
Background:
The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.
Background References:
  • Laifenfeld, D., Karry, R., Grauer, E., Klein, E., and Ben-Shachar, D. "ATF2, a member of the CREB/ATF family of transcription factors, in chronicstress and consequent to antidepressant treatment: animal models and humanpost-mortem brains." Neuropsychopharmacology, 29 (2004) 589 - 97.
  • Bhoumik, A., Jones, N., and Ronai, Z., "Transcriptional switch by activating transcription factor 2-derived peptide sensitizes melanoma cells to apoptosis and inhibits their tumorigenicity," Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 4222 - 4227.
  • Ivanov, V.N., Bhoumik, A., and Ronai, Z., "Death receptors and melanoma resistance to apoptosis," Oncogene 22 (2003) 3152 - 3161.
  • Gutpa, S., Campbell, D., Derijard, B. and Davis, R. J., "Transcription factor ATF2 regulation by the JNK signal transduction pathway," Science 267 (1995) 389-393.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
ATF-2 (Ser 490/498) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1386
NCBI Official Name:
activating transcription factor 2
NCBI Official Symbol:
ATF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74
Protein Accession #:
P15336
Protein GI Number:
215274241
Purification:
Affinity Purified
Research Area:
Neuroscience ,Signal Transduction ,Phospho-Specific
SPECIFICITY:
ATF2 antibody is specific for ~74k ATF2 phosphorylated at Ser490/498.
Swissprot #:
P15336
User NOte:
Optimal dilutions for each application to be determined by the researcher.