Akt Antibody

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ProSci
Product Code: PSI-79-131
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-131-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, serum or both using Akt (Phospho-Thr308).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, serum or both using Akt (Phospho-Thr308).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).

Documents

Further Information

Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, CFL, C-AKT, PKB-alpha, RAC-PK-alpha
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Background References:
  • Tremblay F, et al. (2005) Diabetes; 54 (9): 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40): 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6): 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt.
Modifications:
Phospho-Specific
NCBI Gene ID #:
207
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol:
AKT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_001014431.1
Protein GI Number:
62241013
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
Swissprot #:
P31749