BAD Antibody
| Code | Size | Price |
|---|
| PSI-79-134-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
BBC2, BCL2L8, ERBB, ERBB1, HER1, BBC6
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Background References:
- Zhang B, et al. (2004) . Mol Cell Biol. 24 (14): 6205-6214. Rice PL, et al. (2003) . Cancer Res. 63 (3): 616-620. Wang XQ, et al. (2001) . J Biol Chem. 276 (48): 44504-44511.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BAD (Phospho-Ser112) antibody was raised against a peptide sequence around phosphorylation site of serine 112 (H-S-S (p) -Y-P) derived from Human BAD.
Modifications:
Phospho-Specific
NCBI Gene ID #:
572
NCBI Official Name:
BCL2-associated agonist of cell death
NCBI Official Symbol:
BAD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
NP_004313.1
Protein GI Number:
10835069
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of BAD only when phosphorylated at serine 112.
Swissprot #:
Q92934