Estrogen Receptor-a Antibody

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ProSci
Product Code: PSI-79-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-137-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-α (Phospho-Ser118).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser118).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Phospho-Ser118).
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Western blot analysis of lysed extracts from MDA cells untreated or treated with Estradiol using Estrogen Receptor-α (Phospho-Ser118).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser118).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Phospho-Ser118).

Documents

Further Information

Additional Names:
ER, ESR, Era, ESRA, ESTRR, NR3A1, BBC6, BCL2L8, ER, ESR1, ESTR, ESTRA
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Background References:
  • Ramsauer VP, et al. (2003) J Biol Chem; 278 (32): 30142-7. Jepson S, et al. (2002) Oncogene; 21 (49): 7524-32. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93. Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19 (3): 645-56.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Estrogen Receptor-a (Phospho-Ser118) antibody was raised against a peptide sequence around phosphorylation site of serine 118 (Q-L-S (p) -P-F) derived from Human Estrogen Receptor-α.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2099
NCBI Official Name:
estrogen receptor 1
NCBI Official Symbol:
ESR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
NP_000116.2
Protein GI Number:
62821794
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Estrogen Receptor-αonly when phosphorylated at serine 118.
Swissprot #:
P03372