Histone H3.1 Antibody

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ProSci
Product Code: PSI-79-190
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-190-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using Histone H3.1 (Phospho-Ser10).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (Phospho-Ser10).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Phospho-Ser10).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using Histone H3.1 (Phospho-Ser10).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (Phospho-Ser10).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Phospho-Ser10).

Documents

Further Information

Additional Names:
H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background References:
  • Dai J, et al. (2005) Genes Dev 19 (4): 472-488. Yih LH, et al. (2005) Carcinogenesis 26 (1): 53-63.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Histone H3.1 (Phospho-Ser10) antibody was raised against a peptide sequence around phosphorylation site of serine 10 (R-K-S (p) -T-G) derived from Human Histone H3.1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
8351
NCBI Official Name:
histone cluster 1, H3d
NCBI Official Symbol:
HIST1H3D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
NP_003521.2
Protein GI Number:
21071021
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Histone H3.1 onlywhen phosphorylated at serine 10.
Swissprot #:
P68431