IkB-a Antibody

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ProSci
Product Code: PSI-79-175
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-175-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).

Documents

Further Information

Additional Names:
IKBA, MAD-3, NFKBI, ADDA, I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBIA
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Background References:
  • Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4792
NCBI Official Name:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
NCBI Official Symbol:
NFKBIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
NP_065390.1
Protein GI Number:
10092619
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36.
Swissprot #:
P25963