SRA Monoclonal Antibody

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ProSci
Product Code: PSI-32-219
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-219-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1D4H8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
SRA monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein human ovary cancer tissue lysate and A431 cell lysate .
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Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein human ovary cancer tissue lysate and A431 cell lysate .

Documents

Further Information

Additional Names:
Steroid receptor RNA activator protein, SRAP, SRA, STRAA1, pp7684
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Background:
Steroid receptor RNA activator 1 (SRA), with 237-amino acid protein (about 27 kDa), belongs to the growing family of functional non-coding RNAs. SRA was originally described as the first functional noncoding RNA able to specifically coactivate the activity of steroid receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. Its expression is strongly up-regulated in many human tumors of the breast, uterus, and ovary, suggesting a potential role in pathogenesis. Although coactivation of steroid-dependent transcription by SRA is accompanied by a proliferative response, overexpression is not in itself sufficient to induce turmorigenesis.
Background References:
  • Rainer B. Lanz, Steven S. Chua, Niall Barron. Mol. Cell. Biol, Oct 2003; 23: 7163 - 7176.
  • Shilpa Chooniedass-Kothari, Mohammad Kariminia Hamedani, Sandy Troup. Int J Cancer. 2006 Feb 15;118(4):1054-9
  • S. Chooniedass-Kothari, E. Emberley, M. K. Hamedani. FEBS Lett. 2004 May 21;566(1-3):43-7
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant SRA expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
10011
NCBI Official Name:
steroid receptor RNA activator 1
NCBI Official Symbol:
SRA1
NCBI Organism:
Homo sapiens
Protein Accession #:
Q9HD15
Protein GI Number:
74718904
Research Area:
Signal Transduction
Swissprot #:
Q9HD15
User NOte:
Optimal dilutions for each application to be determined by the researcher.