NMDA NR2B Antibody
| Code | Size | Price |
|---|
| PSI-XPS-2025-10ug | 10ug | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
NMDA NR2B Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C and is stable at -20˚C for at least 1 year. Ambient shipment is suitable for both international an
Documents
Further Information
Application Note:
NMDA NR2B Subunit antibody is specific for the 180 kDa NR2B subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2B subunit that was used to generate the antibody. No reactivity towards the NR2A subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Immunolabeling has been demonstrated in human, mouse and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, 1:1000-1:2000 for IHC, and in IP, 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2B.
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer?s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression.
Background References:
- Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther., 97 (2003) 55 - 85.
- Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C., and Browning, M.D., "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat," J. Neurosci., 22 (2002) 3628 - 3637.
- Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci., 5 (2002) 27 - 33.
- Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L., "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion," Mol. Brain Res., 40 (1996) 71 - 78.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
NMDA NR2B polyclonal antibody was raised against a C-terminal fusion protein of the NR2B subunit of the NMDAR.
NCBI Gene ID #:
24410
NCBI Official Name:
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol:
Grin2b
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
PREDICTED MOLECULAR WEIGHT:
180
Protein Accession #:
Q00960
Protein GI Number:
548372
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
Q00960
User NOte:
Optimal dilutions for each application to be determined by the researcher.