DARPP-32 Antibody

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ProSci
Product Code: PSI-50-201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-201-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Chicken (Gallus)
  • Frog
  • Human
  • Mouse
  • Rat
  • Zebrafish
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
DARPP-32 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
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Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, rat, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac? (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Background References:
  • Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671.
  • Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.
  • Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774-778
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
NCBI Gene ID #:
360616
NCBI Official Name:
protein phosphatase 1, regulatory (inhibitor) subunit 1B
NCBI Official Symbol:
Ppp1r1b
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32
Protein Accession #:
Q6J4I0
Protein GI Number:
81884861
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
Q6J4I0
User NOte:
Optimal dilutions for each application to be determined by the researcher.