Dopamine Transporter Antibody

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ProSci
Product Code: PSI-50-205
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-50-205-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Dopamine Transporter antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.
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Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.

Documents

Further Information

Application Note:
The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.
Background:
The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
Background References:
  • Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
  • Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.
  • Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).
NCBI Gene ID #:
6531
NCBI Official Name:
solute carrier family 6 (neurotransmitter transporter), member 3
NCBI Official Symbol:
SLC6A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88
Protein Accession #:
Q01959
Protein GI Number:
266667
Purification:
Affinity Purified
Research Area:
Neuroscience
Swissprot #:
Q01959
User NOte:
Optimal dilutions for each application to be determined by the researcher.