GABAA Receptor Antibody

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ProSci
Product Code: PSI-50-269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-269-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
GABAA Receptor (Ser327) antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex showing specific immunolabeling of the ~45k GABAA γ 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
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Western blot of rat cortex showing specific immunolabeling of the ~45k GABAA γ 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Documents

Further Information

Background:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gamma;s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKC? regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)
Background References:
  • Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
  • Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdell?s B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
  • Ogris W, Poltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ2-subunit. Biochem Pharmacol 68:1621-1629.
  • Qi ZH, Song M, Wallace MJ, Wang D, Newton PM, McMahon T, Chou WH, Zhang C, Shokat KM, Messing RO (2007) Protein kinase Cε regulates γ-aminobutyrate type A receptor sensitivity
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of the GABAA receptor, y2 subunit.
Modifications:
Phospho-Specific
NCBI Gene ID #:
29709
NCBI Official Name:
gamma-aminobutyric acid (GABA) A receptor, gamma 2
NCBI Official Symbol:
Gabrg2
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45
Protein Accession #:
P18508
Protein GI Number:
120788
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
Specific for ~45k GABAA receptor ?2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by ?-phosphatase treatment.
Swissprot #:
P18508
User NOte:
Optimal dilutions for each application to be determined by the researcher.