Tau Antibody

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ProSci
Product Code: PSI-50-270
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-270-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Tau (Ser416) antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
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Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Documents

Further Information

Background:
Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer?s disease (AD), Pick?s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).
Background References:
  • Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Bio Oct; 101(4):1371-8.
  • Lee V.M.Y. (1995) Disruption of the cytoskeleton in Alzheimer?s disease. Curr. Opin. Neurobiol. 5, 663-668.
  • Goedert M. and Jakes R. (1990) Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization. EMBO J 9, 4225-4230.
  • Steiner B., Mandelkow E.M., Biernat J. et al. (1990) Phosphorylation of microtubule-associated protein tau: identification of the site for Ca2+-calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles. EMBO J. 9, 3539-3544.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of rat tau.
Modifications:
Phospho-Specific
NCBI Gene ID #:
29477
NCBI Official Name:
microtubule-associated protein tau
NCBI Official Symbol:
Mapt
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59/65/68
Protein Accession #:
P19332
Protein GI Number:
13432197
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
Specific for ~59, 65, 68k tau protein phosphorylated at Ser416.
Swissprot #:
P19332
User NOte:
Optimal dilutions for each application to be determined by the researcher.