Neurofilament NF-H Antibody

Contact us
ProSci
Product Code: PSI-50-254
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-254-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Neurofilament NF-H antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

1 / 1
Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. <br><br> <b>Below:</b> Immunofluorescence of rat cortical neurons and glia showing NF-H staining (red).
❮❯

Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. <br><br> <b>Below:</b> Immunofluorescence of rat cortical neurons and glia showing NF-H staining (red).

Documents

Further Information

Background:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
Background References:
  • Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
  • Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933.
  • Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Purified bovine NF-H.
NCBI Gene ID #:
4744
NCBI Official Name:
neurofilament, heavy polypeptide
NCBI Official Symbol:
NEFH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
200
Protein Accession #:
P12036
Protein GI Number:
226726294
Purification:
Total IgY fraction
Research Area:
Neuroscience
SPECIFICITY:
Specific for the ~200k Neurofilament H protein.
Swissprot #:
P12036
User NOte:
Optimal dilutions for each application to be determined by the researcher.