Glial Fibrillary Acidic Protein Antibody

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ProSci
Product Code: PSI-50-268
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-268-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Glial Fibrillary Acidic Protein antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein. <br><br> <b>Below:</b> Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
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Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein. <br><br> <b>Below:</b> Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Documents

Further Information

Background:
Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers.
Background References:
  • Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
  • Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001)
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant and purified bovine GFAP.
NCBI Gene ID #:
281189
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Bos taurus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50
Protein Accession #:
Q28115
Protein GI Number:
143811396
Purification:
Total IgY fraction
Research Area:
Neuroscience
SPECIFICITY:
Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
Swissprot #:
Q28115
User NOte:
Optimal dilutions for each application to be determined by the researcher.