Neurofilament NF-M Monoclonal Antibody

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ProSci
Product Code: PSI-50-257
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-257-0.1mL0.1mL£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Avian
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Neurofilament NF-M antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein.
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Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein.

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Further Information

Background:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
Background References:
  • Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
  • Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
NCBI Gene ID #:
24588
NCBI Official Name:
neurofilament, medium polypeptide
NCBI Official Symbol:
Nefm
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
145
Protein Accession #:
P12839
Protein GI Number:
128150
Purification:
Total IgG fraction
Research Area:
Neuroscience
SPECIFICITY:
Specific for the ~145k Neurofilament M protein.
Swissprot #:
P12839
User NOte:
Optimal dilutions for each application to be determined by the researcher.