ATF2 Antibody

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ProSci
Product Code: PSI-79-116
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-116-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from Huvec cells untreated or treated with Anisomycin using ATF2 (Phospho-Ser112 or 94).
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Ser112 or 94).
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Western blot analysis of lysed extracts from Huvec cells untreated or treated with Anisomycin using ATF2 (Phospho-Ser112 or 94).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Ser112 or 94).

Documents

Further Information

Additional Names:
HB16, CREB2, TREB7, CREB-2, CRE-BP1, CDKN2, CREBP1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Background References:
  • Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATF2 (Phospho-Ser112 or 94) antibody was raised against a peptide sequence around phosphorylation site of serine 112 or 94 (D-L-S (p) -P-L) derived from Human ATF2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1386
NCBI Official Name:
activating transcription factor 2
NCBI Official Symbol:
ATF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 - 75 kDa
Protein Accession #:
NP_001871.2
Protein GI Number:
22538422
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of ATF-2 only when phosphorylated at serine 112 or 94.
Swissprot #:
P15336