DARPP 32 Antibody

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ProSci
Product Code: PSI-XPS-1004
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1004-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚

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Western blot of rat caudate showign phospho-specific immunolabeing of the ~32k DARPP-32 protein phosphorylated at Thr34.
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Western blot of rat caudate showign phospho-specific immunolabeing of the ~32k DARPP-32 protein phosphorylated at Thr34.

Documents

Further Information

Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr34) recognizes human, mouse, and rat forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac?.
Background References:
  • Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway," Science, 302 (2003) 1412 - 1415.
  • Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.
  • Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens," Nature Neurosci. 5 (2002) 641 - 648.
  • Bibb, J.A., Snyder, G.L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A.A., Tsai, L.H., Kwon, Y.T., Girault, J.A., Czernik, A.J., Huganir, R.L., Hemmings, Jr., H.C., Nairn, A.C., and Greengard, P., "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons, Nature (London) 402 (1999) 669 - 671.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
DARPP-32 (Thr34) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr34 of DARPP-32.
Modifications:
Phospho-Specific
NCBI Gene ID #:
360616
NCBI Official Name:
protein phosphatase 1, regulatory (inhibitor) subunit 1B
NCBI Official Symbol:
Ppp1r1b
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32
Protein Accession #:
Q6J4I0
Protein GI Number:
81884861
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
DARPP 32 antibody is specific for the ~32k DARPP-32 phosphorylated at Thr34.
Swissprot #:
Q6J4I0
User NOte:
Optimal dilutions for each application to be determined by the researcher.