Synaptotagmin Antibody

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ProSci
Product Code: PSI-XPS-1028
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1028-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

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Synaptotagmin (Phospho-Ser309) Antibody
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Synaptotagmin (Phospho-Ser309) Antibody

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Further Information

Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.
Background References:
  • Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo," Nature (London) 426 (2003) 559 - 563.
  • Wang, C.T., Lu, J.C., Bai, J.H., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion," Nature (London) 424 (2003) 943 - 947.
  • Fernandez-Chacon, R., Konigstorfer, A., Gerber, S.H., Garcia, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Sudhof, T.C., "Synaptotagmin I functions as a calcium regulator of release probability," Nature (London ) 410 (2001) 41 - 49.
  • Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P., and Czernik, A.J., "Regulation of synaptotagmin I phosphorylation by multiple protein kinases," J. Neurochem. 73 (1999) 921 - 932.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Synaptotagmin (Ser309) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser309 of synaptotagmin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
25716
NCBI Official Name:
synaptotagmin I
NCBI Official Symbol:
Syt1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60/62
Protein Accession #:
P21707
Protein GI Number:
94730428
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
Synaptotagmin antibody is specific for Mr 60 - 62k synaptotagmin phosphorylated at Ser309.
Swissprot #:
P21707
User NOte:
Optimal dilutions for each application to be determined by the researcher.