Tyrosine Hydroxylase Antibody

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ProSci
Product Code: PSI-XPS-2033
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-2033-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Sheep
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

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Tyrosine Hydroxylase, Sheep Antibody
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Tyrosine Hydroxylase, Sheep Antibody

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Further Information

Application Note:
Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB). The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
Background:
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.
Background References:
  • Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies," Neuroscience, 82 (1998) 727-738.
  • Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies," J. Neurochem. 60 (1993) 493-502.
  • Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla," J. Neurochem. 56 (1991) 2139 - 2142.
  • Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody," Anal. Biochem. 181 (1989) 259 - 266.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.
NCBI Gene ID #:
25085
NCBI Official Name:
tyrosine hydroxylase
NCBI Official Symbol:
Th
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60
Protein Accession #:
P04177
Protein GI Number:
136577
Purification:
Affinity Purified
Research Area:
Neuroscience ,Signal Transduction
Swissprot #:
P04177
User NOte:
Optimal dilutions for each application to be determined by the researcher.