Tyrosine Hydroxylase Antibody
| Code | Size | Price |
|---|
| PSI-XPS-1032-0.1mL | 0.1mL | £686.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Documents
Further Information
Application Note:
Applications include Dot Blots (DB), Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.
Background:
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.
Background References:
- Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
- Salvatore, M.F., Garcia-Espana, A., Goldstein, M., Deutch, A.Y., and Haycock, J.W., "Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds," J. Neurochem., 75 (2000) 225 - 232.
- Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies," Neuroscience, 82 (1998) 727-738.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
TH (Ser31) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser31 of rat tyrosine hydroxylase (TH).
Modifications:
Phospho-Specific
NCBI Gene ID #:
25085
NCBI Official Name:
tyrosine hydroxylase
NCBI Official Symbol:
Th
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60
Protein Accession #:
P04177
Protein GI Number:
136577
Purification:
Affinity Purified
Research Area:
Neuroscience ,Signal Transduction ,Phospho-Specific
SPECIFICITY:
Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser31 in PC-12 cells stimulated by Okadaic Acid.
Swissprot #:
P04177
User NOte:
Optimal dilutions for each application to be determined by the researcher.