Tyrosine Hydroxylase Antibody

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ProSci
Product Code: PSI-XPS-1033
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-XPS-1033-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Porcine (pig)
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western Blots of phospho- and dephospho-proteins to demonstrate the selectivity of the Phospho-Specific Antibody. The Pan-Specific Antibody recognized both the dephospho-Tyrosine Hydroxylase (dephospho-TH) and the phospho-Tyrosine Hydroxylase (phospho-TH). Most importantly, the Phospho-Specific Antibody recognized only phospho-TH whereas the Dephospho-Specific Antibody reacted selectively with dephospho-TH.<br><br><b>Below:</b>:Immunohistochemical staining of retina with the Pan-Tyrosine Hydroxylase (Pan-TH) and Phospho-Specific Tyrosine Hydroxylase (Phospho-TH) Antibodies. The Pan-TH Antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the Phospho-TH Antibody selectively labels only the two amacrine cells in this light-stimulated retina example
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Western Blots of phospho- and dephospho-proteins to demonstrate the selectivity of the Phospho-Specific Antibody. The Pan-Specific Antibody recognized both the dephospho-Tyrosine Hydroxylase (dephospho-TH) and the phospho-Tyrosine Hydroxylase (phospho-TH). Most importantly, the Phospho-Specific Antibody recognized only phospho-TH whereas the Dephospho-Specific Antibody reacted selectively with dephospho-TH.<br><br><b>Below:</b>:Immunohistochemical staining of retina with the Pan-Tyrosine Hydroxylase (Pan-TH) and Phospho-Specific Tyrosine Hydroxylase (Phospho-TH) Antibodies. The Pan-TH Antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the Phospho-TH Antibody selectively labels only the two amacrine cells in this light-stimulated retina example

Documents

Further Information

Application Note:
Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
Background:
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.
Background References:
  • Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
  • Haycock, J. W., Lew, J. Y., Garcia-Espana, A., Lee, K. Y., Harada, K., Meller, E., and Goldstein, M., "Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis," J. Neurochem., 71 (1998) 1670-1675.
  • Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies," Neuroscience, 82 (1998) 727-738.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH).
Modifications:
Phospho-Specific
NCBI Gene ID #:
25085
NCBI Official Name:
tyrosine hydroxylase
NCBI Official Symbol:
Th
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60
Protein Accession #:
P04177
Protein GI Number:
136577
Purification:
Affinity Purified
Research Area:
Neuroscience ,Signal Transduction ,Phospho-Specific
SPECIFICITY:
Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 ?M for 60 minutes). Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.
Swissprot #:
P04177
User NOte:
Optimal dilutions for each application to be determined by the researcher.