GPR125 Antibody
| Code | Size | Price |
|---|
| PSI-46-851-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
GPR125 , G protein-coupled receptor 125 , PGR21, TEM5L, TEM5-like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml. A customer reported successful results on Human Testis.
Background References:
- Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, Scherr DS, Zhang F, Torres R, Gale NW, Yancopoulos GD, Murphy A, Valenzuela DM, Hobbs RM, Pandolfi PP, Rafii S. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature. 2007 Sep 20;449(7160):346-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: CTNKSASSFKNS
NCBI Gene ID #:
166647
NCBI Official Name:
G protein-coupled receptor 125
NCBI Official Symbol:
GPR125
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_660333.2
Protein GI Number:
59823631
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
Q8IWK6