KCNB1 Antibody
| Code | Size | Price |
|---|
| PSI-46-678-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
KCNB1, potassium voltage-gated channel, Shab-related subfamily, member 1, DRK1, KV2.1, h-DRK1, delayed rectifier potassium channel Kv2.1, h-DRK1 K channel, potassium channel protein DRK1, potassium voltage-gated channel subfamily B member 1, voltage-gated potassium channel
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Park KS, Mohapatra DP, Misonou H, Trimmer JS. Graded regulation of the Kv2.1 potassium channel by variable phosphorylation. Science. 2006 Aug 18;313(5789):976-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-HQYIDADTDDEGQ
NCBI Gene ID #:
3745
NCBI Official Name:
potassium voltage-gated channel, Shab-related subfamily, member 1
NCBI Official Symbol:
KCNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004966.1
Protein GI Number:
4826784
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
Q14721