GFP Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-160-0.1mL | 0.1mL | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4B10B2
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
GFP monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Green fluorescent protein
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Background References:
- Dittman JS. Kaplan JM. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. Epub 2006 Jul 14.
- Cavalieri V. Bernardo MD. Spinelli G. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. Epub 2006 Jun 14.
- Arasada R. Son H. Ramalingam N. et al. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68. Epub 2006 Jul 13.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
7011691
NCBI Official Name:
green fluorescent protein
NCBI Official Symbol:
gfp
NCBI Organism:
Neisseria gonorrhoeae
Protein Accession #:
YP_002302326.1
Protein GI Number:
211909965
Research Area:
Phospho-Specific
Swissprot #:
B6UPG7
User NOte:
Optimal dilutions for each application to be determined by the researcher.