RCAN1 Antibody

ProSci
Product Code: PSI-43-007
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-007-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-007 (1ug/ml) staining of Mouse Brain and KO Mouse Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-007 (1ug/ml) staining of Mouse Brain and KO Mouse Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
RCAN1, regulator of calcineurin 1, ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1, Down syndrome candidate region 1, Down syndrome critical region gene 1, OTTHUMP00000108621, OTTHUMP00000108622, OTTHUMP00000214669, OTTHUMP00000214670, calcipressin-1, calcium and oxidant-inducible mRNA, down syndrome critical region protein 1, modulatory calcineurin-interacting protein 1, myocyte-enriched calcineurin-interacting protein 1, near DSCR proline-rich protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they represent non-specific signal. We call for caution when used for other assays than Western blot. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA. Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HIGSSHLAPPNPD
NCBI Gene ID #:
1827
NCBI Official Name:
regulator of calcineurin 1
NCBI Official Symbol:
RCAN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 40 kDa
Protein Accession #:
NP_004405.3, NP_981962.1, NP_981963.1
Protein GI Number:
33620725
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all three reported isoforms (NP_004405.3; NP_981962.1; NP_981963.1).
Swissprot #:
P53805