DSS1 Antibody

ProSci
Product Code: PSI-45-500
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-500-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8KDa according to NP_006295. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Background References:
  • Wei SJ, Trempus CS, Cannon RE, Bortner CD, Tennant RW. Identification of Dss1 as a 12-O-Tetradecanoylphorbol-13-acetate-responsive Gene Expressed in Keratinocyte Progenitor Cells, with Possible Involvement in Early Skin Tumorigenesis. J Biol Chem. 2003 Jan 17;278(3):1758-68.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-RAELEKHGYKMETS
NCBI Gene ID #:
7979
NCBI Official Name:
split hand/foot malformation (ectrodactyly) type 1
NCBI Official Symbol:
SHFM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006295.1
Protein GI Number:
5453640
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P60896