DTX1 Antibody

ProSci
Product Code: PSI-42-248
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-248-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
DTX1, deltex homolog 1 (Drosophila), hDx-1, deltex homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 48kDa band in Mouse Fetal Brain lysates and in lysates of NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.4kDa according to Human NP_004407.2 and of 68.2kDa according to Mouse NP_032078.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Gupta-Rossi N, Storck S, Griebel PJ, Reynaud CA, Weill JC, Dahan A, Specific over-expression of deltex and a new Kelch-like protein in human germinal center B cells. Molecular immunology 2003 Mar 39 (13): 791-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CHHIENVLKEDAR
NCBI Gene ID #:
1840
NCBI Official Name:
deltex 1, E3 ubiquitin ligase
NCBI Official Symbol:
DTX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004407.2
Protein GI Number:
41352718
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Cancer
Swissprot #:
Q86Y01