PEBP4 Antibody

ProSci
Product Code: PSI-42-236
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-236-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-236 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-236 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PEBP4, phosphatidylethanolamine-binding protein 4, CORK-1, CORK1, GWTM1933, MGC22776, PRO4408, cousin-of-RKIP 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Heart lysates (calculated MW of 25.7kDa according to NP_659399.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10ug/ml.
Background References:
  • Trynka G, Zhernakova A, Romanos J, Franke L, Hunt K, Turner G, Platteel M, Ryan AW, de Kovel C, Barisani D, Bardella MT, McManus R, Van Heel DA, Wijmenga C. Coeliac disease associated risk variants in TNFAIP3 and REL implicate altered NF-{kappa}B signalling. Gut. 2009 Feb 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RERASEPKHKNQAE
NCBI Gene ID #:
157310
NCBI Official Name:
phosphatidylethanolamine-binding protein 4
NCBI Official Symbol:
PEBP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_659399.2
Protein GI Number:
116812622
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q96S96