DTX3 Antibody
Code | Size | Price |
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PSI-42-249-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
DTX3, deltex 3 homolog (Drosophila), FLJ34766, MGC138863, MGC138864, RNF154, deltex 3 homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 10ug/ml.
Background References:
- Takeyama K, Aguiar RC, Gu L, He C, Freeman GJ, Kutok JL, Aster JC, Shipp MA, The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity. The Journal of biological chemistry 2003 Jun 278 (24): 21930-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-KNKVTVSKPVWD
NCBI Gene ID #:
196403
NCBI Official Name:
deltex 3, E3 ubiquitin ligase
NCBI Official Symbol:
DTX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_848597.1
Protein GI Number:
30425428
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q8N9I9